TY - JOUR T1 - A cross-package Bioconductor workflow for analysing methylation array data JF - bioRxiv DO - 10.1101/055087 SP - 055087 AU - Jovana Maksimovic AU - Belinda Phipson AU - Alicia Oshlack Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/05/25/055087.abstract N2 - Methylation in the human genome is known to be associated with development and disease. The Illumina Infinium methylation arrays are by far the most common way to interrogate methylation across the human genome. This paper provides a Bioconductor workflow using multiple packages for the analysis of methylation array data. Specifically, we demonstrate the steps involved in a typical differential methylation analysis pipeline including: quality control, filtering, normalization, data exploration and statistical testing for probe-wise differential methylation. We further outline other analyses such as differential methylation of regions, differential variability analysis, estimating cell type composition and gene ontology testing. Finally, we provide some examples of how to visualise methylation array data. ER -