RT Journal Article
SR Electronic
T1 The Evolution of CHROMOMETHYLASES and Gene Body DNA Methylation in Plants
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 054924
DO 10.1101/054924
A1 Adam J. Bewick
A1 Chad E. Niederhuth
A1 Nicholas A. Rohr
A1 Patrick T. Griffin
A1 Jim Leebens-Mack
A1 Robert J. Schmitz
YR 2016
UL http://biorxiv.org/content/early/2016/05/25/054924.abstract
AB The evolution of gene body methylation (gbM) and the underlying mechanism is poorlyunderstood. By pairing the largest collection of CHROMOMETHYLASE (CMT) sequences (773)and methylomes (72) across land plants and green algae we provide novel insights into the evolution of gbM and its underlying mechanism. The angiosperm-and eudicot-specific whole genome duplication events gave rise to what are now referred to as CMT1, 2 and3 lineages. CMTε, which includes the eudicot-specific CMT1 and 3, and orthologous angiosperm clades, is essential for the perpetuation of gbM in angiosperms, implyinthat gbM evolved at least 236 MYA. Independent losses of CMT1, 2 and 3 in eudicots, and CMT2 and CMTεmonocot+magnoliid in monocots suggests overlapping orfluid functional evolution. The resulting gene family phylogeny of CMT transcripts from the most diverse sampling of plants to date redefines our understanding of CMT evolution and its evolutionary consequences on DNA methylation.