TY - JOUR T1 - Deriving shape-based features for <em>C. elegans</em> locomotion using dimensionality reduction methods JF - bioRxiv DO - 10.1101/054379 SP - 054379 AU - Bertalan Gyenes AU - André E.X. Brown Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/05/19/054379.abstract N2 - High-throughput analysis of animal behavior has become a reality with the advance of recording technology, leading to large high-dimensional data sets. This dimensionality can sometimes be reduced while still retaining relevant information. In the case of the nematode worm Caenorhabditis elegans, more than 90% of the shape variance can be captured using just four principal components. However, it remains unclear if other methods can achieve a more compact representation or contribute further biological insight to worm locomotion. Here we take a data-driven approach to worm shape analysis using independent component analysis (ICA), non-negative matrix factorization (NMF), a cosine series, and jPCA and confirm that the dimensionality of worm shape space is close to four. Projecting worm shapes onto the bases derived using each method gives interpretable features ranging from head movements to tail oscillation. We use these as a comparison method to find differences between the wild type N2 worms and various mutants. The different bases provide complementary views of worm behavior and we expect that closer examination of the time series of projected amplitudes will lead to new results in the future. ER -