RT Journal Article
SR Electronic
T1 Differentiation-dependent telomeric long non-coding transcription in a model of skeletal myogenesis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 000679
DO 10.1101/000679
A1 Scott W. Brouilette
A1 Samir Ounzain
A1 Vinit Sawhney
A1 Kenta Yashiro
A1 Yasunori Shintani
A1 Kunihiko Takahashi
A1 Steven R. Coppen
A1 Takuya Narita
A1 Kelli Torsney
A1 Martin Carrier
A1 Niall Campbell
A1 Ken Suzuki
YR 2013
UL http://biorxiv.org/content/early/2013/11/18/000679.abstract
AB Telomeres comprise the distal ends of eukaryotic chromosomes, serve to maintain genomic integrity and are extended by the ribonucleoprotein telomerase. Recent evidence indicates that telomeres are transcribed to generate long non-coding RNAs (lncRNAs) and that these transcripts (TERRA) may inhibit telomerase activity. In this study we assessed telomerase activity and telomeric lncRNA expression in a mouse model of skeletal myogenesis. Using the C2C12 cell line we demonstrated decreased telomerase activity during differentiation into terminally-differentiated skeletal myotubes. Despite existing in a post-mitotic state, residual telomerase activity remained in C2C12 myotubes, indicating a role independent of telomere extension. Telomeric transcripts were detected in both myoblasts and myotubes, with reduced expression during differentiation correlating with reduced telomerase expression. Our data indicate that in a mouse model of skeletal myogenesis TERRA expression does not reduce telomerase activity, suggesting that their relationship is more complex than originally perceived; the role of telomeric derived lncRNAs in relation to telomerase activity may be cell-type specific. These findings raise the possibility for novel non-telomerase regulatory function for TERRA-lncRNAs during skeletal myogenesis.