RT Journal Article
SR Electronic
T1 Goggatomy: a Method for Opening Small Cuticular Compartments in Arthropods for Physiological Experiments
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 053298
DO 10.1101/053298
A1 Alan R. Kay
A1 Davide Raccuglia
A1 Jon Scholte
A1 Elena Loukianova
A1 Christopher Barwacz
A1 Steven R. Armstrong
A1 C. Allan Guymon
A1 Michael N. Nitabach
A1 Daniel F. Eberl
YR 2016
UL http://biorxiv.org/content/early/2016/05/18/053298.abstract
AB Most sense organs of arthropods are ensconced in small exoskeletal compartments that hinder direct access to plasma membranes. We have developed a method for exposing live sensory and supporting cells in such structures. The technique uses a viscous light cured resin to embed and support the structure, which is then sliced with a sharp blade. We term the procedure a ‘goggatomy’, from the Khoisan word for a bug, gogga. To demonstrate the utility of the method we show that it can be used to expose the auditory chordotonal organs in the second antennal segment and the olfactory receptor neurons in the third antennal segment of Drosophila melanogaster, preserving the transduction machinery. The procedure can also be used on other small arthropods, like mites, Daphnia, mosquitoes, wasps and ants to expose a variety of cells.