RT Journal Article
SR Electronic
T1 Genome sequencing of a single tardigrade Hypsibius dujardini individual
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 053223
DO 10.1101/053223
A1 Kazuharu Arakawa
A1 Yuki Yoshida
A1 Masaru Tomita
YR 2016
UL http://biorxiv.org/content/early/2016/05/13/053223.abstract
AB Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.