PT  - JOURNAL ARTICLE
AU  - Kazuharu Arakawa
AU  - Yuki Yoshida
AU  - Masaru Tomita
TI  - Genome sequencing of a single tardigrade Hypsibius dujardini individual
AID  - 10.1101/053223
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 053223
4099  - http://biorxiv.org/content/early/2016/05/13/053223.short
4100  - http://biorxiv.org/content/early/2016/05/13/053223.full
AB  - Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.