RT Journal Article
SR Electronic
T1 The relative values of the turnover number and the dissociation rate constant determine the definition of the Michaelis-constant
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 052514
DO 10.1101/052514
A1 Gassan Nazzal
YR 2016
UL http://biorxiv.org/content/early/2016/05/12/052514.abstract
AB The Km value is widely used and defined as a measure of the stability of the ES-complex, however, this is true if the first reaction step exists in rapid equilibrium and the reaction is activation controlled. This paper will discuss the theoretical limitation of each case of the quasi-steady-state approximation using the statistical thermodynamic and the diffusion theory to define the Km value.