TY - JOUR T1 - High-throughput functional annotation of influenza A virus genome at single-nucleotide resolution JF - bioRxiv DO - 10.1101/005702 SP - 005702 AU - Nicholas C. Wu AU - Arthur P. Young AU - Laith Q. Al-Mawsawi AU - Anders C. Olson AU - Jun Feng AU - Hangfei Qi AU - Shu-Hwa Chen AU - I-Hsuan Lu AU - Chung-Yen Lin AU - Robert G. Chin AU - Harding H. Luan AU - Nguyen Nguyen AU - Stanley F. Nelson AU - Xinmin Li AU - Ting-Ting Wu AU - Ren Sun Y1 - 2014/01/01 UR - http://biorxiv.org/content/early/2014/05/31/005702.abstract N2 - A novel genome-wide genetics platform is presented in this study, which permits functional interrogation of all point mutations across a viral genome in parallel. Here we generated the first fitness profile of individual point mutations across the influenza virus genome. Critical residues on the viral genome were systematically identified, which provided a collection of subdomain data informative for structure-function studies and for effective rational drug and vaccine design. Our data was consistent with known, well-characterized structural features. In addition, we have achieved a validation rate of 68% for severely attenuated mutations and 94% for neutral mutations. The approach described in this study is applicable to other viral or microbial genomes where a means of genetic manipulation is available. ER -