@article {Wu005702, author = {Nicholas C. Wu and Arthur P. Young and Laith Q. Al-Mawsawi and Anders C. Olson and Jun Feng and Hangfei Qi and Shu-Hwa Chen and I-Hsuan Lu and Chung-Yen Lin and Robert G. Chin and Harding H. Luan and Nguyen Nguyen and Stanley F. Nelson and Xinmin Li and Ting-Ting Wu and Ren Sun}, title = {High-throughput functional annotation of influenza A virus genome at single-nucleotide resolution}, elocation-id = {005702}, year = {2014}, doi = {10.1101/005702}, publisher = {Cold Spring Harbor Laboratory}, abstract = {A novel genome-wide genetics platform is presented in this study, which permits functional interrogation of all point mutations across a viral genome in parallel. Here we generated the first fitness profile of individual point mutations across the influenza virus genome. Critical residues on the viral genome were systematically identified, which provided a collection of subdomain data informative for structure-function studies and for effective rational drug and vaccine design. Our data was consistent with known, well-characterized structural features. In addition, we have achieved a validation rate of 68\% for severely attenuated mutations and 94\% for neutral mutations. The approach described in this study is applicable to other viral or microbial genomes where a means of genetic manipulation is available.}, URL = {https://www.biorxiv.org/content/early/2014/05/31/005702}, eprint = {https://www.biorxiv.org/content/early/2014/05/31/005702.full.pdf}, journal = {bioRxiv} }