TY - JOUR T1 - <em>Drosophila</em> poised enhancers are generated during tissue patterning with the help of repression JF - bioRxiv DO - 10.1101/052142 SP - 052142 AU - Nina Koenecke AU - Jeff Johnston AU - Qiye He AU - Julia Zeitlinger Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/05/07/052142.abstract N2 - Histone modifications are frequently used as markers for enhancer states, but how to interpret enhancer states in the context of embryonic development is not clear. The poised enhancer signature, involving H3K4me1 and low levels of H3K27ac, has been reported to mark inactive enhancers that are poised for future activation. However, future activation is not always observed and alternative reasons for the widespread occurrence of this enhancer signature have not been investigated. By analyzing enhancers during dorsal-ventral (DV) axis formation in the Drosophila embryo, we find that the poised enhancer signature is specifically generated during patterning in the tissue where the enhancers are not induced, including at enhancers that are known to be repressed by a transcriptional repressor. These results suggest that, rather than serving simply as an intermediate step before future activation, the poised enhancer state may mark enhancers for spatial activation during tissue patterning. We discuss the possibility that the poised enhancer state is more generally the result of repression by transcriptional repressors. ER -