TY - JOUR T1 - Synthetic lipid-containing scaffolds enhance production by co-localizing enzymes JF - bioRxiv DO - 10.1101/052035 SP - 052035 AU - Cameron Myhrvold AU - Jessica K. Polka AU - Pamela A. Silver Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/05/06/052035.abstract N2 - Subcellular organization is critical for isolating, concentrating, and protecting biological activities. Natural subcellular organization is often achieved using co-localization of proteins on scaffold molecules, thereby enhancing metabolic fluxes and enabling co-regulation. Synthetic scaffolds extend these benefits to new biological processes, and are typically constructed from proteins or nucleic acids. To expand the range of available building materials, we use a minimal set of components from the lipid-encapsulated bacteriophage Φ6 to form synthetic lipid-containing scaffolds (SLSs) in E. coli. Analysis of diffusive behavior by tracking particles in live cells indicates that SLSs are >20 nm in diameter; furthermore, density measurements demonstrate that SLSs contain a mixture of lipids and proteins. The fluorescent proteins mCitrine and mCerulean can be co-localized to SLSs. To test for effects on enzymatic production, we localized two enzymes involved in indigo biosynthesis to SLSs. We observed a scaffold-dependent increase in indigo production, showing that SLSs can enhance metabolic reactions. ER -