@article {Myhrvold052035, author = {Cameron Myhrvold and Jessica K. Polka and Pamela A. Silver}, title = {Synthetic lipid-containing scaffolds enhance production by co-localizing enzymes}, elocation-id = {052035}, year = {2016}, doi = {10.1101/052035}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Subcellular organization is critical for isolating, concentrating, and protecting biological activities. Natural subcellular organization is often achieved using co-localization of proteins on scaffold molecules, thereby enhancing metabolic fluxes and enabling co-regulation. Synthetic scaffolds extend these benefits to new biological processes, and are typically constructed from proteins or nucleic acids. To expand the range of available building materials, we use a minimal set of components from the lipid-encapsulated bacteriophage Φ6 to form synthetic lipid-containing scaffolds (SLSs) in E. coli. Analysis of diffusive behavior by tracking particles in live cells indicates that SLSs are \>20 nm in diameter; furthermore, density measurements demonstrate that SLSs contain a mixture of lipids and proteins. The fluorescent proteins mCitrine and mCerulean can be co-localized to SLSs. To test for effects on enzymatic production, we localized two enzymes involved in indigo biosynthesis to SLSs. We observed a scaffold-dependent increase in indigo production, showing that SLSs can enhance metabolic reactions.}, URL = {https://www.biorxiv.org/content/early/2016/05/06/052035}, eprint = {https://www.biorxiv.org/content/early/2016/05/06/052035.full.pdf}, journal = {bioRxiv} }