RT Journal Article
SR Electronic
T1 Calcium imaging reveals coordinated simple spike pauses in populations of Cerebellar Purkinje cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 051730
DO 10.1101/051730
A1 Jorge E. Ramirez
A1 Brandon M. Stell
YR 2016
UL http://biorxiv.org/content/early/2016/05/04/051730.abstract
AB The brain’s control of movement is thought to involve coordinated activity between cerebellar Purkinje cells. The results reported here demonstrate that somatic Ca2+ imaging is a faithful reporter of Na+-dependent “simple spike” pauses and enables us to optically record changes in firing rates in populations of Purkinje cells. This simultaneous calcium imaging of populations of Purkinje cells reveals a striking spatial organization of pauses in Purkinje cell activity between neighboring cells. The source of this organization is shown to be the presynaptic GABAergic network and blocking GABAARs abolishes the synchrony. These data suggest that presynaptic interneurons synchronize (in)activity between neighboring Purkinje cells and thereby maximize their effect on downstream targets in the deep cerebellar nuclei.