TY - JOUR T1 - Calcium imaging reveals coordinated simple spike pauses in populations of Cerebellar Purkinje cells JF - bioRxiv DO - 10.1101/051730 SP - 051730 AU - Jorge E. Ramirez AU - Brandon M. Stell Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/05/04/051730.abstract N2 - The brain’s control of movement is thought to involve coordinated activity between cerebellar Purkinje cells. The results reported here demonstrate that somatic Ca2+ imaging is a faithful reporter of Na+-dependent “simple spike” pauses and enables us to optically record changes in firing rates in populations of Purkinje cells. This simultaneous calcium imaging of populations of Purkinje cells reveals a striking spatial organization of pauses in Purkinje cell activity between neighboring cells. The source of this organization is shown to be the presynaptic GABAergic network and blocking GABAARs abolishes the synchrony. These data suggest that presynaptic interneurons synchronize (in)activity between neighboring Purkinje cells and thereby maximize their effect on downstream targets in the deep cerebellar nuclei. ER -