RT Journal Article
SR Electronic
T1 System-wide quantitative proteomics of the metabolic syndrome in mice: genotypic and dietary effects
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 051508
DO 10.1101/051508
A1 Camille Terfve
A1 Eduard Sabidó
A1 Yibo Wu
A1 Emanuel Gonçalves
A1 Meena Choi
A1 Stefania Vaga
A1 Olga Vitek
A1 Julio Saez-Rodriguez
A1 Ruedi Aebersold
YR 2016
UL http://biorxiv.org/content/early/2016/05/03/051508.abstract
AB Advances in mass spectrometry have made the quantitative measurement of proteins across multiple samples a reality, allowing for the study of complex biological systems such as the metabolic syndrome. Although deregulation of lipid metabolism and increased hepatic storage of triacylglycerides are known to play a part in the onset of the metabolic syndrome, its molecular basis and dependency on dietary and genotypic factors are poorly characterized. Here, we used a rich experimental design with two different mouse strains, dietary and metabolic perturbations to generate a compendium of quantitative proteome data, using three mass spectrometry strategies. The data recapitulates known properties of the metabolic system and indicate differential molecular adaptation of the two mouse strains to perturbations, contributing to a better understanding of the metabolic syndrome. We show that high-quality, high-throughput proteomic datasets provide an unbiased broad overview of complex systems upon perturbation.HIGHLIGHTSRich experimental design with two mouse strains, dietary and metabolic perturbationsThree mass spectrometry proteomic data collection strategies in one data compendiumSWATH-MS data with systematic perturbation provide a broad unbiased view of the systemThe data reveals differential adaptation of molecular systems in two mouse strains