RT Journal Article SR Electronic T1 Sub-atomic resolution X-ray diffraction of the SH3 domain from the post-synaptic density protein Shank3 JF bioRxiv FD Cold Spring Harbor Laboratory SP 051425 DO 10.1101/051425 A1 Srinivas Kumar Ponna A1 Matti Myllykoski A1 Tobias M. Boeckers A1 Petri Kursula YR 2016 UL http://biorxiv.org/content/early/2016/05/02/051425.abstract AB The post-synaptic density multidomain scaffolding proteins of the Shank family are structurally poorly characterised. The Shank family consists of three members, and domain-specific interactions of Shank are involved in forming a network of proteins at the post-synaptic region for intracellular signalling and cellular scaffolding. While X-ray crystallography has provided some information on individual Shank domains, the structural basis of Shank interactions is still largely unknown. In this study, the production and crystallisation of the previously uncharacterised Shank3 SH3 domain is presented. The highly twinned crystals diffracted synchrotron X-rays to a resolution higher than 0.9 Å, and these crystals will eventually have the potential to provide an ultrahigh-resolution view into the Shank family SH3 domains and their interactions.