RT Journal Article
SR Electronic
T1 Carbon nanotube electrodes for retinal implants: a study of structural and functional integration over time
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 050633
DO 10.1101/050633
A1 Cyril G. Eleftheriou
A1 Jonas B. Zimmermann
A1 Henrik D. Kjeldsen
A1 Moshe David-Pur
A1 Yael Hanein
A1 Evelyne Sernagor
YR 2016
UL http://biorxiv.org/content/early/2016/04/27/050633.abstract
AB The choice of electrode material is of paramount importance in neural prosthetic devices. Electrodes must be biocompatible yet able to sustain repetitive current injections in a highly corrosive environment. We explored the suitability of carbon nanotube (CNT) electrodes to stimulate retinal ganglion cells (RGCs) in a mouse model of outer retinal degeneration. We investigated morphological changes at the bio-hybrid interface and changes in RGC responses to electrical stimulation following prolonged in vitro coupling to CNT electrodes. We observed gradual remodelling of the inner retina to incorporate CNT assemblies. Electrophysiological recordings demonstrate a progressive increase in coupling between RGCs and the CNT electrodes over three days, characterized by a gradual decrease in stimulation thresholds and increase in cellular recruitment. These results provide novel evidence for time-dependent formation of viable bio-hybrids between CNTs and the retina, demonstrating that CNTs are a promising material for inclusion in retinal prosthetic devices.