RT Journal Article
SR Electronic
T1 Charting Improvements in US Registry HLA Typing Ambiguity Using a Typing Resolution Score
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 050443
DO 10.1101/050443
A1 Vanja Paunić
A1 Loren Gragert
A1 Joel Schneider
A1 Carlheinz Müller
A1 Martin Maiers
YR 2016
UL http://biorxiv.org/content/early/2016/04/26/050443.abstract
AB Unrelated stem cell registries have been collecting HLA typing of volunteer bone marrow donors for over 25 years. Donor selection for hematopoietic stem cell transplantation is based primarily on matching the alleles of donors and patients at five polymorphic HLA loci. As HLA typing technologies have continually advanced since the beginnings of stem cell transplantation, registries have accrued typings of varied HLA typing ambiguity. We present a new typing resolution score, based on the likelihood of self-match, that allows the systematic comparison of HLA typings across different methods, data sets and populations. We apply the typing resolution score to chart improvement in HLA typing within the Be The Match Registry of the United States from the initiation of DNA-based HLA typing to the current state of high-resolution typing using next-generation sequencing technologies. In addition, we present a publicly available online tool for evaluation of any given HLA typing. This typing resolution score objectively evaluates HLA typing methods and can help define standards for acceptable recruitment HLA typing.