RT Journal Article SR Electronic T1 Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta JF bioRxiv FD Cold Spring Harbor Laboratory SP 050286 DO 10.1101/050286 A1 Ray Yu-Ruei Wang A1 Yifan Song A1 Benjamin A Barad A1 Yifan Cheng A1 James S Fraser A1 Frank DiMaio YR 2016 UL http://biorxiv.org/content/early/2016/04/26/050286.abstract AB Cryo-EM has revealed many challenging yet exciting macromolecular assemblies at near-atomic resolution (3-4.5Å), providing biological phenomena with molecular descriptions. However, at these resolutions accurately positioning individual atoms remains challenging and may be error-prone. Manually refining thousands of amino acids – typical in a macromolecular assembly – is tedious and time-consuming. We present an automated method that can improve the atomic details in models manually built in near-atomic-resolution cryo-EM maps. Applying the method to three systems recently solved by cryo-EM, we are able to improve model geometry while maintaining or improving the fit-to-density. Backbone placement errors are automatically detected and corrected, and the refinement shows a large radius of convergence. The results demonstrate the method is amenable to structures with symmetry, of very large size, and containing RNA as well as covalently bound ligands. The method should streamline the cryo-EM structure determination process, providing accurate and unbiased atomic structure interpretation of such maps.