RT Journal Article
SR Electronic
T1 Ex-STORM: Expansion Single Molecule Super-resolution Microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 049403
DO 10.1101/049403
A1 Zhisong Tong
A1 Paolo Beuzer
A1 Qing Ye
A1 Josh Axelrod
A1 Zhenmin Hong
A1 Hu Cang
YR 2016
UL http://biorxiv.org/content/early/2016/04/25/049403.abstract
AB We present critical improvements to recently invented Expansion Microscopy (ExM), which resolve the incompatibility between ExM and single molecule super-resolution microscopy STORM. Specifically, the improved ExM circumvents the massive, 50-100%, bleaching of fluorophores during sample preparations, and preserve the efficiency of enzymatic oxygen scavenging systems in expanded samples. These improvements open up new avenues for Ex-STORM - expanding a sample mechanically, and then visualizing the sample with STORM.