%0 Journal Article %A Zhisong Tong %A Paolo Beuzer %A Qing Ye %A Josh Axelrod %A Zhenmin Hong %A Hu Cang %T Ex-STORM: Expansion Single Molecule Super-resolution Microscopy %D 2016 %R 10.1101/049403 %J bioRxiv %P 049403 %X We present critical improvements to recently invented Expansion Microscopy (ExM), which resolve the incompatibility between ExM and single molecule super-resolution microscopy STORM. Specifically, the improved ExM circumvents the massive, 50-100%, bleaching of fluorophores during sample preparations, and preserve the efficiency of enzymatic oxygen scavenging systems in expanded samples. These improvements open up new avenues for Ex-STORM - expanding a sample mechanically, and then visualizing the sample with STORM. %U https://www.biorxiv.org/content/biorxiv/early/2016/04/25/049403.full.pdf