RT Journal Article SR Electronic T1 Epigenetic control of phenotypic plasticity in a filamentous fungus Neurospora crassa JF bioRxiv FD Cold Spring Harbor Laboratory SP 049726 DO 10.1101/049726 A1 Ilkka Kronholm A1 Hanna Johannesson A1 Tarmo Ketola YR 2016 UL http://biorxiv.org/content/early/2016/04/22/049726.abstract AB Phenotypic plasticity is the ability of a genotype to produce different phenotypes under different environmental or developmental conditions. Phenotypic plasticity is an ubiquitous feature of living organisms, and is typically based on variable patterns of gene expression. However, the mechanisms by which gene expression is influenced and regulated during plastic responses are poorly understood in most organisms. While modifications to DNA and histone proteins have been implicated as likely candidates for generating and regulating phenotypic plasticity, specific details of each modification and its mode of operation have remained largely unknown. In this study, we investigated how epigenetic mechanisms affect phenotypic plasticity in the filamentous fungus Neurospora crassa. By measuring reaction norms of strains that are deficient in one of several key physiological processes we show that epigenetic mechanisms play a role in homeostasis and phenotypic plasticity of the fungus across a range of controlled environments. Effects on plasticity are specific to an environment and mechanism, indicating that epigenetic regulation is context dependent and is not governed by general plasticity genes. In our experiments with Neurospora, histone methylation and the RNA interference pathway had the greatest influence on phenotypic plasticity, while lack of DNA methylation had the least.