PT  - JOURNAL ARTICLE
AU  - Zhisong Tong
AU  - Paolo Beuzer
AU  - Qing Ye
AU  - Josh Axelrod
AU  - Zhenmin Hong
AU  - Hu Cang
TI  - ExM-STORM: Expansion Single Molecule Nanoscopy
AID  - 10.1101/049403
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 049403
4099  - http://biorxiv.org/content/early/2016/04/20/049403.short
4100  - http://biorxiv.org/content/early/2016/04/20/049403.full
AB  - Recently, Chen et al. invented Expansion Microscopy, or ExM, which imprints a specimen’s features of interest onto a polymer hydrogel network and expands the network homogeneously to magnify sub-diffraction-limit features. However, ExM bleaches massively, 50-100% of the fluorescent molecules; thus it is not suited for single-molecule imaging. Here, we describe improved ExM that is free of bleaching, and thus can be synergically integrated with single-molecule nanoscopy, STORM.