TY - JOUR T1 - Low base-substitution mutation rate in the germline genome of the ciliate <em>Tetrahymena thermophila</em> JF - bioRxiv DO - 10.1101/025536 SP - 025536 AU - Hongan Long AU - David J. Winter AU - Allan Y.-C. Chang AU - Way Sun AU - Steven H. Wu AU - Mariel Balboa AU - Ricardo B.R. Azevedo AU - Reed A. Cartwright AU - Michael Lynch AU - Rebecca A. Zufall Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/04/20/025536.abstract N2 - Mutation is the ultimate source of all genetic variation and is, therefore, central to evolutionary change. Previous work on Paramecium tetraurelia found an unusually low germline base-substitution mutation rate in this ciliate. Here, we tested the generality of this result among ciliates using Tetrahymena thermophila. We sequenced the genomes of 10 lines of T. thermophila that had each undergone approximately 1,000 generations of mutation accumulation (MA). We developed a new probabilistic mutation detection approach that directly models the design of an MA experiment and accommodates the noise introduced by mismapped reads and also applied an existing mutation-calling pipeline. From these methods, we find that T. thermophila has a germline base-substitution mutation rate of 7.61 × 10−12 per site, per cell division, which is consistent with the low base-substitution mutation rate in P. tetraurelia. Over the course of the evolution experiment, genomic exclusion lines derived from the MA lines experienced a fitness decline that cannot be accounted for by germline base-substitution mutations alone, suggesting that other genetic or epigenetic factors must be involved. Because selection can only operate to reduce mutation rates based upon the “visible” mutational load, asexual reproduction with a transcriptionally silent germline may allow ciliates to evolve extremely low germline mutation rates. ER -