RT Journal Article
SR Electronic
T1 The Impact of African and Brazilian Zika virus isolates on neuroprogenitors
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 046599
DO 10.1101/046599
A1 Loraine Campanati
A1 Luiza M. Higa
A1 Rodrigo Delvecchio
A1 Paula Pezzuto
A1 Ana Luiza Valadão
A1 Fábio L. Monteiro
A1 Grasiella M. Ventura
A1 Carla Veríssimo
A1 Ana M. Bispo De Filippis
A1 Renato S. Aguiar
A1 Amilcar Tanuri
YR 2016
UL http://biorxiv.org/content/early/2016/04/20/046599.abstract
AB In the last few months, an overwhelming number of people have been exposed to the Zika virus (ZIKV) in South and Central America. Here we showed, in vitro, that a Brazilian isolate impacts more severely murine neuronal progenitors and neurons than the African strain MR766. We found that the Brazilian isolate more pronouncedly inhibits neurite extension from neurospheres, alters their differentiation potential and causes neurons to have less and shorter processes. Comparing both lineages using a panel of inflammatory cytokines, we showed, with human neuroblastoma cells, that ZIKV induces the production of several inflammatory and chemotactic cytokines and once again, the Brazilian isolate had a more significant impact. Although much more needs to be studied regarding the association of ZIKV infection and brain damage during development, our study sheds some light into the differences between African and American lineages and the mechanisms by which the virus may be affecting neurogenesis.