RT Journal Article SR Electronic T1 Impact of knowledge accumulation on pathway enrichment analysis JF bioRxiv FD Cold Spring Harbor Laboratory SP 049288 DO 10.1101/049288 A1 Lina Wadi A1 Mona Meyer A1 Joel Weiser A1 Lincoln D. Stein A1 Jüri Reimand YR 2016 UL http://biorxiv.org/content/early/2016/04/19/049288.abstract AB Pathway-based interpretation of gene lists is a staple of genome analysis. It depends on frequently updated gene annotation databases. We analyzed the evolution of gene annotations over the past seven years and found that the vocabulary of pathways and processes has doubled. This strongly impacts practical analysis of genes: 80% of publications we surveyed in 2015 used outdated software that only captured 20% of pathway enrichments apparent in current annotations.