TY - JOUR T1 - Unsupervised spike sorting for large scale, high density multielectrode arrays JF - bioRxiv DO - 10.1101/048645 SP - 048645 AU - Gerrit Hilgen AU - Martino Sorbaro AU - Sahar Pirmoradian AU - Jens-Oliver Muthmann AU - Ibolya E. Kepiro AU - Simona Ullo AU - Cesar Juarez Ramirez AU - Alessandro Maccione AU - Luca Berdondini AU - Vittorio Murino AU - Diego Sona AU - Francesca Cella Zanacchi AU - Upinder S. Bhalla AU - Evelyne Sernagor AU - Matthias H Hennig Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/04/13/048645.abstract N2 - A new method for automated spike sorting for recordings with high density, large scale multielectrode arrays is presented. It is based on an efficient, low-dimensional representation of detected events by their estimated spatial current source locations and dominant spike shape features. Millions of events can be sorted in just minutes, and the full analysis chain scales roughly linearly with recording time. We demonstrate this method using recordings from the mouse retina with a 4,096 channel array, and present validation based on anatomical imaging and model-based quality control. Our analysis shows that it is feasible to reliably isolate the activity of hundreds to thousands of retinal ganglion cells in single recordings. ER -