RT Journal Article
SR Electronic
T1 Third-generation sequencing and the future of genomics
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 048603
DO 10.1101/048603
A1 Hayan Lee
A1 James Gurtowski
A1 Shinjae Yoo
A1 Maria Nattestad
A1 Shoshana Marcus
A1 Sara Goodwin
A1 W. Richard McCombie
A1 Michael C. Schatz
YR 2016
UL http://biorxiv.org/content/early/2016/04/13/048603.abstract
AB Third-generation long-range DNA sequencing and mapping technologies are creating a renaissance in high-quality genome sequencing. Unlike second-generation sequencing, which produces short reads a few hundred base-pairs long, third-generation single-molecule technologies generate over 10,000 bp reads or map over 100,000 bp molecules. We analyze how increased read lengths can be used to address longstanding problems in de novo genome assembly, structural variation analysis and haplotype phasing.