RT Journal Article
SR Electronic
T1 Ultra fast tissue staining with chemical tags
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 005298
DO 10.1101/005298
A1 Johannes Kohl
A1 Julian Ng
A1 Sebastian Cachero
A1 Michael-John Dolan
A1 Ben Sutcliffe
A1 Daniel Krueger
A1 Shahar Frechter
A1 Gregory S.X.E. Jefferis
YR 2014
UL http://biorxiv.org/content/early/2014/05/19/005298.abstract
AB Genetically encoded fluorescent proteins and immunostainings are widely used to detect cellular or subcellular structures in thick biological samples. However, each approach suffers from limitations, including low signal and limited spectral flexibility or slow speed, poor penetration and high background, respectively. Here we overcome these limitations by using transgenically expressed chemical tags for rapid, even and low-background labeling of thick biological tissues. We construct a platform of widely applicable transgenic Drosophila reporter lines, demonstrating that chemical labeling can accelerate staining of whole-mount fly brains by a factor of 100×. Together, this tag-based approach drastically improves the speed and specificity of labeling genetically marked cells in intact and/or thick biological samples.