RT Journal Article SR Electronic T1 Insights into telomerase action from high-throughput sequencing of S. pombe telomeres JF bioRxiv FD Cold Spring Harbor Laboratory SP 047837 DO 10.1101/047837 A1 Henrietta W. Bennett A1 Na Liu A1 Yan Hu A1 Megan C. King YR 2016 UL http://biorxiv.org/content/early/2016/04/09/047837.abstract AB We have developed a high-throughput sequencing approach that enables us to determine terminal telomere sequences from tens of thousands of individual Schizosaccharomyces pombe telomeres. This method provides unprecedented coverage of telomeric sequence complexity in fission yeast. S. pombe telomeres are composed of modular degenerate repeats that can be explained by variation in usage of the TER1 RNA template during reverse transcription. Taking advantage of this deep sequencing approach, we find that “like” repeat modules are highly correlated within individual telomeres. Moreover, repeat module preference varies with telomere length, suggesting that existing repeats promote the incorporation of like repeats and/or that specific conformations of the telomerase holoenzyme efficiently and/or processively add repeats of like nature. After the loss of telomerase activity, this sequencing and analysis pipeline defines a population of telomeres with altered sequence content. This approach should be adaptable to study telomeric repeats in other organisms and can provide new insights into telomere sequence content at high density.