RT Journal Article SR Electronic T1 Generation of Aggregates of Mouse ES Cells that Show Symmetry Breaking, Polarisation and Emergent Collective Behaviour in vitro JF bioRxiv FD Cold Spring Harbor Laboratory SP 005215 DO 10.1101/005215 A1 Peter Baillie-Johnson A1 Susanne C. van den Brink A1 Tina Balayo A1 David A. Turner A1 Alfonso Martinez Arias YR 2014 UL http://biorxiv.org/content/early/2014/05/15/005215.abstract AB Dissociated mouse embryonic stem (ES) cells were cultured to form aggregates in small volumes of basal medium in U-bottomed, non tissue-culture-treated 96-well plates and subsequently maintained in suspension culture. After growth for 48 hours, the aggregates are competent to respond to ubiquitous experimental signals which result in their symmetry-breaking and generation of defined polarised structures by 96 hours. It is envisaged that this system can be applied both to the study of early developmental events and more broadly to the processes of self-organisation and cellular decision-making. It may also provide a suitable niche for the generation of cell types present in the embryo but unobtainable from conventional adherent culture.ChiCHIR99021DMH1Dorsomorphin-H1ES cellEmbryonic Stem CellPD03PD0325901SB43SB431542