PT - JOURNAL ARTICLE AU - Melissa A. McAlexander AU - Kenneth W. Witwer TI - Synthetic miR-16-5p does not act as a reverse transcriptase co-factor to enhance detection of small RNA AID - 10.1101/047001 DP - 2016 Jan 01 TA - bioRxiv PG - 047001 4099 - http://biorxiv.org/content/early/2016/04/04/047001.short 4100 - http://biorxiv.org/content/early/2016/04/04/047001.full AB - Failure to detect low-abundance microRNAs (miRNAs), for example, in circulating plasma, may occur for a variety of reasons, including presence of enzyme inhibitors. Recently, we received the unusual but intriguing suggestion that miR-16-5p acts as a co-factor of reverse transcriptases, facilitating more efficient reverse transcription of miRNAs and thus enhanced detection of low-abundance miRNAs. We tested this hypothesis by incubating reverse transcriptase with several concentrations of synthetic miR-16-5p and then performing stem-loop RT-qPCR with serial dilutions of miRNA osa-MIR168a. Our results do not support a role for miR-16 as a co-factor of reverse transcriptase.