TY - JOUR T1 - Expansion of the CRISPR toolbox in an animal with tRNA-flanked Cas9 and Cpf1 gRNAs JF - bioRxiv DO - 10.1101/046417 SP - 046417 AU - Fillip Port AU - Simon L Bullock Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/03/31/046417.1.abstract N2 - We present vectors for producing multiple CRISPR gRNAs from a single RNA polymerase II or III transcript in Drosophila. The system, which is based on liberation of gRNAs by processing of flanking t †RNAs, permits highly efficient multiplexing of Cas9-based mutagenesis. We also demonstrate that the †RNA-gRNA system markedly increases the efficacy of conditional gene disruption by Cas9 and can promote editing by the recently discovered RNA-guided endonuclease Cpf1. ER -