PT  - JOURNAL ARTICLE
AU  - Fillip Port
AU  - Simon L Bullock
TI  - Expansion of the CRISPR toolbox in an animal with tRNA-flanked Cas9 and Cpf1 gRNAs
AID  - 10.1101/046417
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 046417
4099  - http://biorxiv.org/content/early/2016/03/31/046417.short
4100  - http://biorxiv.org/content/early/2016/03/31/046417.full
AB  - We present vectors for producing multiple CRISPR gRNAs from a single RNA polymerase II or III transcript in Drosophila. The system, which is based on liberation of gRNAs by processing of flanking t †RNAs, permits highly efficient multiplexing of Cas9-based mutagenesis. We also demonstrate that the †RNA-gRNA system markedly increases the efficacy of conditional gene disruption by Cas9 and can promote editing by the recently discovered RNA-guided endonuclease Cpf1.