RT Journal Article
SR Electronic
T1 CLAMP directly interacts with MSL2 to facilitate <em>Drosophila</em> dosage compensation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 415455
DO 10.1101/415455
A1 Evgeniya Tikhonova
A1 Anna Fedotova
A1 Artem Bonchuk
A1 Vladic Mogila
A1 Erica N. Larschan
A1 Pavel Georgiev
A1 Oksana Maksimenko
YR 2018
UL http://biorxiv.org/content/early/2018/09/12/415455.abstract
AB The binding of Drosophila male-specific lethal (MSL) dosage compensation complex exclusively to male X chromosome provides an excellent model system to understand mechanisms of selective recruitment of protein complexes to chromatin. Previous studies showed that the male-specific organizer of the complex, MSL2, and ubiquitous DNA-binding protein CLAMP are key players in the specificity of X chromosome binding. The CXC domain of MSL2 binds to genomic sites of MSL complex recruitment. Here we demonstrated that MSL2 directly interacts with the N-terminal zinc-finger domain of CLAMP. CLAMP-MSL2 and CXC-DNA interactions are cooperatively involved in recruitment of MSL complex to the X chromosome.