RT Journal Article SR Electronic T1 Div-Seq: A single nucleus RNA-Seq method reveals dynamics of rare adult newborn neurons in the CNS JF bioRxiv FD Cold Spring Harbor Laboratory SP 045989 DO 10.1101/045989 A1 Naomi Habib A1 Yinqing Li A1 Matthias Heidenreich A1 Lukasz Swiech A1 John J. Trombetta A1 Feng Zhang A1 Aviv Regev YR 2016 UL http://biorxiv.org/content/early/2016/03/27/045989.abstract AB Transcriptomes of individual neurons provide rich information about cell types and dynamic states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation from dense adult tissue is challenging, and markers for each phase are limited. Here, we developed Div-Seq, which combines Nuc-Seq, a scalable single nucleus RNA-Seq method, with EdU-mediated labeling of proliferating cells. We first show that Nuc-Seq can sensitively identify closely related cell types within the adult hippocampus. We apply Div-Seq to track transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Finally, we find rare adult newborn GABAergic neurons in the spinal cord, a non-canonical neurogenic region. Taken together, Nuc-Seq and Div-Seq open the way for unbiased analysis of any complex tissue.