PT  - JOURNAL ARTICLE
AU  - Naomi Habib
AU  - Yinqing Li
AU  - Matthias Heidenreich
AU  - Lukasz Swiech
AU  - John J. Trombetta
AU  - Feng Zhang
AU  - Aviv Regev
TI  - Div-Seq: A single nucleus RNA-Seq method reveals dynamics of rare adult newborn neurons in the CNS
AID  - 10.1101/045989
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 045989
4099  - http://biorxiv.org/content/early/2016/03/27/045989.short
4100  - http://biorxiv.org/content/early/2016/03/27/045989.full
AB  - Transcriptomes of individual neurons provide rich information about cell types and dynamic states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation from dense adult tissue is challenging, and markers for each phase are limited. Here, we developed Div-Seq, which combines Nuc-Seq, a scalable single nucleus RNA-Seq method, with EdU-mediated labeling of proliferating cells. We first show that Nuc-Seq can sensitively identify closely related cell types within the adult hippocampus. We apply Div-Seq to track transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Finally, we find rare adult newborn GABAergic neurons in the spinal cord, a non-canonical neurogenic region. Taken together, Nuc-Seq and Div-Seq open the way for unbiased analysis of any complex tissue.