RT Journal Article SR Electronic T1 fluff: exploratory analysis and visualization of high-throughput sequencing data JF bioRxiv FD Cold Spring Harbor Laboratory SP 045526 DO 10.1101/045526 A1 Georgios Georgiou A1 Simon J. van Heeringen YR 2016 UL http://biorxiv.org/content/early/2016/03/24/045526.abstract AB Summary Here we introduce fluff, a software package that allows for simple exploration, clustering and visualization of high-throughput sequencing experiments. The package contains three command-line tools to generate publication-quality figures in an uncomplicated manner using sensible defaults. Genome-wide data can be aggregated, clustered and visualized in a heatmap, according to different clustering methods. This includes a predefined setting to identify dynamic clusters between different conditions or developmental stages. Alternatively, clustered data can be visualized in a bandplot. Finally, fluff includes a tool to generate genomic profiles. As command-line tools, the fluff programs can easily be integrated into standard analysis pipelines. The installation is straightforward and documentation is available at http://fluff.readthedocs.org.Availability fluff is implemented in Python and runs on Linux. The source code is freely available for download at https://github.com/simonvh/fluff.Contact s.vanheeringen{at}science.ru.nl