TY - JOUR T1 - <em>Drosophila</em> learn efficient paths to a food source JF - bioRxiv DO - 10.1101/033969 SP - 033969 AU - Rapeechai Navawongse AU - Deepak Choudhury AU - Marlena Raczkowska AU - James Charles Stewart AU - Terrence Lim AU - Mashiur Rahman AU - Alicia Guek Geok Toh AU - Zhiping Wang AU - Adam Claridge-Chang Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/03/21/033969.abstract N2 - Elucidating the genetic, and neuronal bases for learned behavior is a central problem in neuroscience. A leading system for neurogenetic discovery is the vinegar fly Drosophila melanogaster; fly memory research has identified genes and circuits that mediate aversive and appetitive learning. However, methods to study adaptive food-seeking behavior in this animal have lagged decades behind rodent feeding analysis, largely due to the challenges presented by their small scale. There is currently no method to dynamically control flies’ access to food. In rodents, protocols that use dynamic food delivery are a central element of experimental paradigms that date back to the influential work of Skinner. This method is still commonly used in the analysis of learning, memory, addiction, feeding, and many other subjects in experimental psychology. The difficulty of microscale food delivery means this is not a technique used in fly behavior. In the present manuscript we describe a microfluidic chip integrated with machine vision and automation to dynamically control defined liquid food presentations and sensory stimuli. Strikingly, repeated presentations of food at a fixed location produced improvements in path efficiency during food approach. This shows that improved path choice is a learned behavior. Active control of food availability using this microfluidic system is a valuable addition to the methods currently available for the analysis of learned feeding behavior in flies. ER -