TY - JOUR T1 - MOHCA-seq: RNA 3D models from single multiplexed proximity-mapping experiments JF - bioRxiv DO - 10.1101/004556 SP - 004556 AU - Clarence Yu Cheng AU - Fang-Chieh Chou AU - Wipapat Kladwang AU - Siqi Tian AU - Pablo Cordero AU - Rhiju Das Y1 - 2014/01/01 UR - http://biorxiv.org/content/early/2014/04/25/004556.abstract N2 - Large RNAs control myriad biological processes but challenge tertiary structure determination. We report that integrating multiplexed •OH cleavage analysis with tabletop deep sequencing (MOHCA-seq) gives nucleotide-resolution proximity maps of RNA structure from single straightforward experiments. After achieving 1-nm resolution models for RNAs of known structure, MOHCA-seq reveals previously unattainable 3D information for ligand-induced conformational changes in a double glycine riboswitch and the sixth community-wide RNA puzzle, an adenosylcobalamin riboswitch. ER -