RT Journal Article
SR Electronic
T1 MOHCA-seq: RNA 3D models from single multiplexed proximity-mapping experiments
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 004556
DO 10.1101/004556
A1 Clarence Yu Cheng
A1 Fang-Chieh Chou
A1 Wipapat Kladwang
A1 Siqi Tian
A1 Pablo Cordero
A1 Rhiju Das
YR 2014
UL http://biorxiv.org/content/early/2014/04/25/004556.abstract
AB Large RNAs control myriad biological processes but challenge tertiary structure determination. We report that integrating multiplexed •OH cleavage analysis with tabletop deep sequencing (MOHCA-seq) gives nucleotide-resolution proximity maps of RNA structure from single straightforward experiments. After achieving 1-nm resolution models for RNAs of known structure, MOHCA-seq reveals previously unattainable 3D information for ligand-induced conformational changes in a double glycine riboswitch and the sixth community-wide RNA puzzle, an adenosylcobalamin riboswitch.