@article {Cheng004556, author = {Clarence Yu Cheng and Fang-Chieh Chou and Wipapat Kladwang and Siqi Tian and Pablo Cordero and Rhiju Das}, title = {MOHCA-seq: RNA 3D models from single multiplexed proximity-mapping experiments}, elocation-id = {004556}, year = {2014}, doi = {10.1101/004556}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Large RNAs control myriad biological processes but challenge tertiary structure determination. We report that integrating multiplexed {\textbullet}OH cleavage analysis with tabletop deep sequencing (MOHCA-seq) gives nucleotide-resolution proximity maps of RNA structure from single straightforward experiments. After achieving 1-nm resolution models for RNAs of known structure, MOHCA-seq reveals previously unattainable 3D information for ligand-induced conformational changes in a double glycine riboswitch and the sixth community-wide RNA puzzle, an adenosylcobalamin riboswitch.}, URL = {https://www.biorxiv.org/content/early/2014/04/25/004556}, eprint = {https://www.biorxiv.org/content/early/2014/04/25/004556.full.pdf}, journal = {bioRxiv} }