TY - JOUR T1 - <em>Drosophila</em> CLAMP is an essential protein with sex-specific roles in males and females JF - bioRxiv DO - 10.1101/042820 SP - 042820 AU - Jennifer A. Urban AU - Caroline A. Doherty AU - William T. Jordan III AU - Jacob E. Bliss AU - Jessica Feng AU - Marcela M. Soruco AU - Leila E. Rieder AU - Erica N. Larschan Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/03/08/042820.abstract N2 - Dosage compensation is a fundamental mechanism in many species that corrects for the inherent imbalance in X-chromosome copy number between XY males and XX females. In Drosophila melanogaster, transcriptional output from the single male X-chromosome is equalized to that of XX females by recruitment of the Male Specific Lethal (MSL) complex to specific sequences along the length of the X-chromosome. The initial recruitment of MSL complex to the X-chromosome is dependent on a recently discovered zinc finger protein called Chromatin-Linked Adapter for MSL Proteins (CLAMP). However, further studies on the in vivo function of CLAMP remained difficult because the location of the gene in pericentric heterochromatin made it challenging to create null mutations or deficiencies. Using the CRISPR/Cas9 genome editing system, we generated the first null mutant in the clamp gene that eliminates expression of CLAMP protein. We show that CLAMP is necessary for both male and female viability. While females die at the third instar larval stage, males die earlier, likely due to the essential role of CLAMP in male dosage compensation. Moreover, we demonstrate that CLAMP promotes dosage compensation in males and represses key male-specific transcripts involved in sex-determination in females. Our results reveal that CLAMP is an essential protein with dual roles in males and females, which together assure that dosage compensation is a sex-specific process. ER -