TY - JOUR T1 - AC-PCA adjusts for confounding variation and recovers the anatomical structure of neocortex JF - bioRxiv DO - 10.1101/040485 SP - 040485 AU - Zhixiang Lin AU - Can Yang AU - Ying Zhu AU - John C. Duchi AU - Yao Fu AU - Yong Wang AU - Bai Jiang AU - Mahdi Zamanighomi AU - Xuming Xu AU - Mingfeng Li AU - Nenad Sestan AU - Hongyu Zhao AU - Wing Hung Wong Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/03/08/040485.abstract N2 - Dimension reduction methods are commonly applied to high-throughput biological datasets. However, the results can be hindered by confounding factors, either biologically or technically originated. In this study, we propose a Principal Component Analysis-based approach to Adjust for Confounding variation (AC-PCA). We show that AC-PCA can adjust for variations across individual donors present in a human brain exon array dataset. Our approach is able to recover the anatomical structure of neocortex regions, including the frontal-temporal and dorsal-ventral axes, and reveal temporal dynamics of the interregional variation. For gene selection purposes, we extend AC-PCA with sparsity constraints, and propose and implement an efficient algorithm. The top selected genes from this algorithm demonstrate frontal/temporal and dorsal/ventral expression gradients and strong functional conservation. ER -