RT Journal Article
SR Electronic
T1 Preprint: A robust peptidomics mass spectrometry platform for measuring oxytocin in plasma and serum
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 042416
DO 10.1101/042416
A1 Ole Kristian Brandtzaeg
A1 Elin Johnsen
A1 Hanne Roberg-Larsen
A1 Knut Fredrik Seip
A1 Siri Leknes
A1 Elsa Lundanes
A1 Steven Ray Wilson
YR 2016
UL http://biorxiv.org/content/early/2016/03/04/042416.abstract
AB Current approaches to measuring the cyclic peptide oxytocin in plasma/serum are associated with poor selectivity and/or inadequate sensitivity. We here describe a high performance nano liquid chromatography-mass spectrometry platform for measuring OT in human plasma/serum. The platform is extremely robust, allowing laborious sample clean-up steps to be omitted. OT binds strongly to plasma proteins, but a reduction/alkylation procedure breaks this bond, allowing ample detection of total OT. The method showed excellent quantitation properties, and was used to determine total OT levels to 0.5-1.2 ng/mL (evaluated with human plasma and cord serum). The method is compatible with accessible mass spectrometry instrumentation, finally allowing selective and easily comparable oxytocin measurements.