RT Journal Article
SR Electronic
T1 <em>In vivo</em> generation of DNA sequence diversity for cellular barcoding
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 004499
DO 10.1101/004499
A1 Ian D. Peikon
A1 Diana I. Gizatullina
A1 Anthony M. Zador
YR 2014
UL http://biorxiv.org/content/early/2014/04/24/004499.abstract
AB Heterogeneity is a ubiquitous feature of biological systems. A complete understanding of such systems requires a method for uniquely identifying and tracking individual components and their interactions with each other. We have developed a novel method of uniquely tagging individual cells in vivo with a genetic “barcode” that can be recovered by DNA sequencing. We demonstrate the feasibility of this technique in bacterial cells. This method should prove useful in tracking interactions of cells within a network, and/or heterogeneity within complex biological samples.