TY - JOUR T1 - Diffusion pseudotime robustly reconstructs lineage branching JF - bioRxiv DO - 10.1101/041384 SP - 041384 AU - Laleh Haghverdi AU - Maren Büttner AU - F. Alexander Wolf AU - Florian Buettner AU - Fabian J. Theis Y1 - 2016/01/01 UR - http://biorxiv.org/content/early/2016/03/02/041384.abstract N2 - Single-cell gene expression profiles of differentiating cells encode their intrinsic latent temporal order. We describe an efficient way to robustly estimate this order according to a diffusion pseudotime, which measures transitions on all length scales between cells using diffusion-like random walks. This allows us to identify cells that undergo branching decisions or are in metastable states, and thereby genes differentially regulated at these states. ER -