RT Journal Article
SR Electronic
T1 Glycolytic enzymes localize to synapses under energy stress to support synaptic function
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 042002
DO 10.1101/042002
A1 SoRi Jang
A1 Jessica C. Nelson
A1 Eric G. Bend
A1 Lucelenie Rodríguez-Laureano
A1 Felipe G. Tueros
A1 Luis Cartagenova
A1 Katherine Underwood
A1 Erik M. Jorgensen
A1 Daniel A. Colón-Ramos
YR 2016
UL http://biorxiv.org/content/early/2016/03/01/042002.abstract
AB Changes in neuronal activity create local and transient changes in energy demands at synapses. Here we discover a metabolic compartment that forms in vivo near synapses to meet local energy demands and support synaptic function in Caenorhabditis elegans neurons. Under conditions of energy stress, glycolytic enzymes redistribute from a diffuse localization in the cytoplasm to a punctate localization adjacent to synapses. Glycolytic enzymes colocalize, suggesting the ad hoc formation of a glycolysis compartment, or a ‘glycolytic metabolon’, that can maintain local levels of ATP. Local formation of the glycolytic metabolon is dependent on presynaptic scaffolding proteins, and disruption of the glycolytic metabolon blocks the synaptic vesicle cycle, impairs synaptic recovery, and affects locomotion. Our studies indicate that energy demands in neurons are met locally through the assembly of a glycolytic metabolon to sustain synaptic function and behavior.