RT Journal Article
SR Electronic
T1 Sensitive red protein calcium indicators for imaging neural activity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 041780
DO 10.1101/041780
A1 Hod Dana
A1 Boaz Mohar
A1 Yi Sun
A1 Sujatha Narayan
A1 Andrew Gordus
A1 Jeremy P. Hasseman
A1 Getahun Tsegaye
A1 Graham T. Holt
A1 Amy Hu
A1 Deepika Walpita
A1 Ronak Patel
A1 John J Macklin
A1 Cornelia I. Bargmann
A1 Misha B. Ahrens
A1 Eric R. Schreiter
A1 Vivek Jayaraman
A1 Loren L. Looger
A1 Karel Svoboda
A1 Douglas S. Kim
YR 2016
UL http://biorxiv.org/content/early/2016/02/29/041780.abstract
AB Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.